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facs staining buffer solution  (Thermo Fisher)


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    Structured Review

    Thermo Fisher facs staining buffer solution
    Facs Staining Buffer Solution, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/facs+staining+buffer+solution/FSB+buffer/pm41525886-178-8-19
    Average 96 stars, based on 1 article reviews
    facs staining buffer solution - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    FACS:

    Article Title: Compartment-specific transcriptome of motor neurons reveals impaired extracellular matrix signaling and activated cell cycle kinases in FUS-ALS.
    Article Snippet: .. Cells were washed and resuspended in 500 μl FACS staining/buffer solution consisting of 500 μl/sample FxCycleTM PI/RNase staining solution (Thermo Fisher Scientific) according to the manufacturer's instructions and incubated in the dark at 37 ◦C for 30 min. .. Finally, flow cytometry measurements were taken at excitation wavelength of 488 nm using a FACSCalibur flow cytometer (BD Biosciences, San Jose, CA, USA).

    Article Title: Hematopoietic stem cell conditioned media induces apoptosis in colorectal cancer stem cells via dysregulation of HSP90 and 26S proteasome system.
    Article Snippet: Cancer stem cells (CSCs) hold a significant role in cancer metastasis, high mortality and severity responsible for therapy resistance and tumour recurrence.. The 26S proteasome system plays a major role in protein degradation in normal cells.. As most cancers have upregulated 26S proteasome machinery, cancer cells use the 26S proteasome system in their favour for growth support by degrading unwanted proteins, but dysfunction of the 26S proteasome system induces apoptosis in cells.

    Article Title: Axonal transcriptome reveals upregulation of PLK1 as a protective mechanism in response to increased DNA damage in FUS P525L spinal motor neurons
    Article Snippet: .. Cells were washed and resuspended in 500 μl FACS staining/buffer solution consisting of 500 μl/sample FxCycleTM PI/RNase staining solution (Thermo Fisher Scientific) according to the manufacturer’s instructions and incubated in the dark at 37 °C for 30 minutes. .. Finally, flow cytometry measurements were taken at excitation wavelength of 488 nm using a FACSCalibur flow cytometer (BD Biosciences, San Jose, CA, USA).

    Staining:

    Article Title: Compartment-specific transcriptome of motor neurons reveals impaired extracellular matrix signaling and activated cell cycle kinases in FUS-ALS.
    Article Snippet: .. Cells were washed and resuspended in 500 μl FACS staining/buffer solution consisting of 500 μl/sample FxCycleTM PI/RNase staining solution (Thermo Fisher Scientific) according to the manufacturer's instructions and incubated in the dark at 37 ◦C for 30 min. .. Finally, flow cytometry measurements were taken at excitation wavelength of 488 nm using a FACSCalibur flow cytometer (BD Biosciences, San Jose, CA, USA).

    Article Title: Hematopoietic stem cell conditioned media induces apoptosis in colorectal cancer stem cells via dysregulation of HSP90 and 26S proteasome system.
    Article Snippet: Cancer stem cells (CSCs) hold a significant role in cancer metastasis, high mortality and severity responsible for therapy resistance and tumour recurrence.. The 26S proteasome system plays a major role in protein degradation in normal cells.. As most cancers have upregulated 26S proteasome machinery, cancer cells use the 26S proteasome system in their favour for growth support by degrading unwanted proteins, but dysfunction of the 26S proteasome system induces apoptosis in cells.

    Article Title: Axonal transcriptome reveals upregulation of PLK1 as a protective mechanism in response to increased DNA damage in FUS P525L spinal motor neurons
    Article Snippet: .. Cells were washed and resuspended in 500 μl FACS staining/buffer solution consisting of 500 μl/sample FxCycleTM PI/RNase staining solution (Thermo Fisher Scientific) according to the manufacturer’s instructions and incubated in the dark at 37 °C for 30 minutes. .. Finally, flow cytometry measurements were taken at excitation wavelength of 488 nm using a FACSCalibur flow cytometer (BD Biosciences, San Jose, CA, USA).

    Incubation:

    Article Title: Compartment-specific transcriptome of motor neurons reveals impaired extracellular matrix signaling and activated cell cycle kinases in FUS-ALS.
    Article Snippet: .. Cells were washed and resuspended in 500 μl FACS staining/buffer solution consisting of 500 μl/sample FxCycleTM PI/RNase staining solution (Thermo Fisher Scientific) according to the manufacturer's instructions and incubated in the dark at 37 ◦C for 30 min. .. Finally, flow cytometry measurements were taken at excitation wavelength of 488 nm using a FACSCalibur flow cytometer (BD Biosciences, San Jose, CA, USA).

    Article Title: Hematopoietic stem cell conditioned media induces apoptosis in colorectal cancer stem cells via dysregulation of HSP90 and 26S proteasome system.
    Article Snippet: Cancer stem cells (CSCs) hold a significant role in cancer metastasis, high mortality and severity responsible for therapy resistance and tumour recurrence.. The 26S proteasome system plays a major role in protein degradation in normal cells.. As most cancers have upregulated 26S proteasome machinery, cancer cells use the 26S proteasome system in their favour for growth support by degrading unwanted proteins, but dysfunction of the 26S proteasome system induces apoptosis in cells.

    Article Title: Axonal transcriptome reveals upregulation of PLK1 as a protective mechanism in response to increased DNA damage in FUS P525L spinal motor neurons
    Article Snippet: .. Cells were washed and resuspended in 500 μl FACS staining/buffer solution consisting of 500 μl/sample FxCycleTM PI/RNase staining solution (Thermo Fisher Scientific) according to the manufacturer’s instructions and incubated in the dark at 37 °C for 30 minutes. .. Finally, flow cytometry measurements were taken at excitation wavelength of 488 nm using a FACSCalibur flow cytometer (BD Biosciences, San Jose, CA, USA).



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    Thermo Fisher facs buffer containing propidium iodide staining solution
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    Thermo Fisher cell sorting facs staining buffer
    A Immunostaining of sagittal sections from a CS17 human embryo. Co-expression of NR2F2 (green) and SOX18 (blue) in the gut. scale bars 50 μm. B-B ”,and C-C ” Sections of proband liver (B) and duodenum (C) stained for CD31 (cyan), SOX18 (red arrows), NR2F2 (green arrows), and <t>DAPI</t> (purple). Orange and gold boxes denote zoomed in regions of individual vessels. Orange box represents vessel that shows cells co-expressing both NR2F2 and SOX18 in the same nucleus, whereas the gold box is a vessel with cells either expressing NR2F2 or SOX18. Scale bar = 50μm (B-C) and 10 μm (B’”-C””).
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    Image Search Results


    A Immunostaining of sagittal sections from a CS17 human embryo. Co-expression of NR2F2 (green) and SOX18 (blue) in the gut. scale bars 50 μm. B-B ”,and C-C ” Sections of proband liver (B) and duodenum (C) stained for CD31 (cyan), SOX18 (red arrows), NR2F2 (green arrows), and DAPI (purple). Orange and gold boxes denote zoomed in regions of individual vessels. Orange box represents vessel that shows cells co-expressing both NR2F2 and SOX18 in the same nucleus, whereas the gold box is a vessel with cells either expressing NR2F2 or SOX18. Scale bar = 50μm (B-C) and 10 μm (B’”-C””).

    Journal: bioRxiv

    Article Title: Inhibiting SOX18 with propranolol restores vascular integrity in NR2F2-driven malformations

    doi: 10.1101/2025.04.25.650344

    Figure Lengend Snippet: A Immunostaining of sagittal sections from a CS17 human embryo. Co-expression of NR2F2 (green) and SOX18 (blue) in the gut. scale bars 50 μm. B-B ”,and C-C ” Sections of proband liver (B) and duodenum (C) stained for CD31 (cyan), SOX18 (red arrows), NR2F2 (green arrows), and DAPI (purple). Orange and gold boxes denote zoomed in regions of individual vessels. Orange box represents vessel that shows cells co-expressing both NR2F2 and SOX18 in the same nucleus, whereas the gold box is a vessel with cells either expressing NR2F2 or SOX18. Scale bar = 50μm (B-C) and 10 μm (B’”-C””).

    Article Snippet: Cells were then washed with FACS buffer (1:5), and resuspended in 300μl FACS buffer + DAPI ((Miltenyi Biotec, # 130-111-570) for live/dead cell identification.

    Techniques: Immunostaining, Expressing, Staining